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KMID : 0380020180330010007
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 1 p.7 ~ p.13
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Fibroin Microsphere-induced 3T3 Cell Migration
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Kwon Se-Chang
Jeon Hyun-Sang
Ryu Da-Yeong
Kim Ji-Young
Hur Won
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Abstract
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Microsphere-stimulated cell migration was observed at the periphery of a confluent 3T3 cell culture even in the absence of serum. Under non-contact inhibition conditions, 3T3 cells showed a highest migratory velocity of 1.7 μm/min in a DMEM media with 0.05 mg/mL microspheres. It is 147% faster than those in DMEM with 10% serum and 194% faster than those in DMEM only. Monolayer colonies were formed from 3T3 cell spheroids in a microsphere-containing culture medium but not in a DMEM without serum. Western blot analysis showed that microsphere supplementation reduced the expression levels of focal adhesion kinase. Fibroin microsphere seems to stimulate a migratory signal cue other than focal adhesion kinase. Unlike to other nanoparticles, fibroin microspheres stimulated 3T3 cell migration, showing a potential candidate for wound healing stimulation.
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KEYWORD
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cell migration, fibroin microsphere, microsphere-stimulation
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